Abcam, ab266109, Human C12orf57 (GRCC10) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
C12orf57 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 1 and Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 1 and Insertion of the selection cassette in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-C12orf57, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GRCC10 also known as G-protein coupled receptor C10 is a transmembrane receptor with a molecular mass of approximately 50 kDa. This protein is expressed in various tissues including the brain liver and lungs. The receptor functions mechanically by binding to specific ligands initiating intracellular signaling cascades. The activation of GRCC10 influences numerous cell processes resulting in different physiological outcomes depending on tissue type.
Biological function summary
GRCC10 plays a significant role in cell communication and signaling. It functions as part of a larger receptor complex which allows it to effectively transmit signals from the extracellular environment to the cell's interior. This signaling affects numerous biological processes such as cell proliferation differentiation and apoptosis. GRCC10's activity is important for maintaining the balance between these cellular outcomes which is important for normal development and function.
Pathways
GRCC10 participates in important signaling networks such as the MAPK/ERK and PI3K/AKT pathways. These pathways control cell growth survival and metabolism. GRCC10 interacts with proteins like RAF and AKT within these pathways helping to modulate their activity. Through its pathway involvement GRCC10 contributes to the regulation of important biological processes including response to hormones and growth factors.
GRCC10 has been linked to conditions like cancer and neurological disorders. Aberrant expression or malfunction of GRCC10 can lead to disease progression often through its interaction with proteins like p53 in cancer or tau in neurodegenerative diseases. Research continues to explore the therapeutic potential of targeting GRCC10 to modulate its role in these disorders.