Abcam, ab266122, Human SLC25A10 (Mitochondrial dicarboxylate carrier) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
SLC25A10 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 7 bp deletion in exon 1 and Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 7 bp deletion in exon 1 and Insertion of the selection cassette in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SLC25A10, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The mitochondrial dicarboxylate carrier also known as the dicarboxylate transporter facilitates the exchange of dicarboxylates across the mitochondrial inner membrane. This carrier protein often termed the dicarboxylate carrier or oxodicarboxylate transporter possesses a molecular mass of approximately 31 kilodaltons and is expressed predominantly in metabolically active tissues such as liver kidney and heart. Its key function lies in transporting four-carbon dicarboxylates like succinate and malate important for energy and metabolic equilibrium.
Biological function summary
The mitochondrial dicarboxylate carrier aids in mitochondrial processes by mediating the shuttle of dicarboxylates important for the tricarboxylic acid (TCA) cycle and urea cycle. It actively participates in the transfer of metabolites that contribute to the synthesis of ATP linking important metabolic routes. The dicarboxylate carrier operates as a part of the family of mitochondrial transport proteins but does not form part of a larger protein complex.
Pathways
The dicarboxylate carrier intersects several key metabolic pathways including the TCA cycle and gluconeogenesis. Through these pathways the protein collaborates with others like the adenine nucleotide translocase; it aids in maintaining energetic homeostasis within cells. The correct functioning of the dicarboxylate carrier ensures the proper flow of carbon skeletons essential for cellular respiration and energy production.
The malfunction of the mitochondrial dicarboxylate carrier links to metabolic conditions like renal tubular acidosis and certain forms of mitochondrial myopathy. The carrier's dysfunction may result in disrupted acid-base homeostasis within the kidneys due to impaired succinate transport. In these contexts proteins such as the mitochondrial pyruvate carrier may exhibit interconnected roles further influencing pathological states. Understanding the carrier's activity impacts strategies for addressing metabolic dysregulations associated with mitochondrial diseases.