Abcam, ab266148, Human ANP32A (PHAP1) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
ANP32A KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 8 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 8 bp deletion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-ANP32A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PHAP1 also known as putative HLA-associated protein 1 or ANP32A is a multifunctional phosphoprotein with a molecular weight of approximately 32 kDa. It exists within the nucleus and cytoplasm indicating its role in various cellular processes. PHAP1 expression is found across diverse tissues showing its widespread functional requirements in different biological contexts.
Biological function summary
PHAP1 plays an important role in regulating apoptosis and cell proliferation. It interacts with components of the INHAT complex which inhibits histone acetylation impacting chromatin remodeling and gene expression. By doing so it influences many cellular events such as cell cycle progression and differentiation. PHAP1's involvement in regulating cell death makes it a target for studying mechanisms underlying cell survival.
Pathways
PHAP1 is associated with apoptosis and cell signaling processes. It is intricately linked with the p38 MAPK signaling pathway important in stress response and inflammation. In these contexts PHAP1 interacts with proteins such as caspases and protein phosphatase 2A which are important for cellular responses to external and internal stimuli.
PHAP1 shows connections with cancer and neurodegenerative diseases. Altered expression levels of PHAP1 have been observed in certain types of cancers possibly affecting tumor growth and treatment responses. In neurodegeneration relations have been reported with tau proteins suggesting PHAP1's role in tauopathies potentially influencing disease progression and providing insights into therapeutic targets.