Abcam, ab266203, Human PPP1R16A knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
PPP1R16A KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 1 and 1 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 1 and 1 bp deletion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PPP1R16A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The PPP1R16A protein also known as myosin light chain phosphatase target subunit 1 is part of the protein phosphatase 1 regulatory subunits. It weighs approximately 84 kDa and is mainly found in tissues like brain and skeletal muscle. It functions as a regulatory subunit modulating the activity of protein phosphatase 1 (PP1) a critical enzyme in cellular signaling.
Biological function summary
This protein plays a significant role in the regulation of myosin phosphatase activity influencing muscle function. As part of a multi-subunit complex PPP1R16A interacts with PP1 to dephosphorylate myosin light chains which is essential for muscle relaxation. This process ensures proper muscle contraction and relaxation cycles in response to cellular signals.
Pathways
Protein phosphatase activity plays key roles in both the smooth muscle contraction and Rho GTPase signaling pathways. PPP1R16A by regulating myosin light chain phosphorylation status interacts with proteins like Rho-associated coiled-coil containing protein kinase (ROCK) within these pathways. This involvement positions PPP1R16A as a vital player in pathways that govern cell motility and muscle contraction.
The dysregulation of PPP1R16A activity is associated with conditions such as hypertension and asthma. Its interaction with ROCK and misregulation in myosin phosphatase activity can contribute to abnormal muscle contractility playing a role in the pathophysiology of these diseases. Understanding this interaction helps in exploring therapeutic targets for related cardiovascular and respiratory disorders.