Abcam, ab266270, Human SDF2L1 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
SDF2L1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 16 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 16 bp deletion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SDF2L1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SDF2L1 also known as stromal cell-derived factor 2-like protein 1 is a protein with a molecular mass of around 38 kDa. Mechanically it functions as a molecular chaperone involved in protein folding within the endoplasmic reticulum (ER). It is expressed in a variety of tissues including the heart liver and kidneys where it plays a role in maintaining protein homeostasis. SDF2L1 aids in the prevention of protein aggregation and assists in the proper folding and assembly of newly synthesized polypeptides in the ER.
Biological function summary
SDF2L1 assists in maintaining ER function during cellular stress conditions. While not part of a large well-defined complex it interacts with multiple proteins to support ER stress responses and the unfolded protein response (UPR). This activity helps protect cells from dysfunction associated with accumulation of misfolded proteins and ensures the degradation or refolding of faulty proteins. As a result SDF2L1 plays a role in cellular adaptation to stress especially during periods of increased protein load.
Pathways
SDF2L1 acts in several interconnected processes involving ER stress and the UPR pathway. It interacts with proteins such as BiP/GRP78 and calnexin which are key components of the UPR. These interactions help modulate the load of unfolded proteins within the ER ensuring that cell survival mechanisms are activated appropriately. SDF2L1's role in these pathways highlights its importance in regulating cellular stress responses and maintaining cellular equilibrium.
Malfunction or dysregulation of SDF2L1 can contribute to the development of conditions like neurodegenerative diseases and liver disorders. Misfolded protein accumulation due to impaired function of SDF2L1 can lead to cellular toxicity which is a characteristic of diseases such as Alzheimer's. SDF2L1 also interacts with proteins like CHOP which is an important mediator of cell apoptosis under chronic ER stress potentially linking it to liver conditions where ER stress is a pathological feature.