Abcam, ab266280, Human MAPK12 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
MAPK12 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 4 and 5 bp deletion in exon 4. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 4 and 5 bp deletion in exon 4

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MAPK12, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The p38 gamma protein also known as MAPK12 is a serine/threonine-protein kinase belonging to the mitogen-activated protein kinase (MAPK) family. It has a molecular mass of approximately 41 kDa. This protein is heavily expressed in skeletal muscle and heart tissues although expression occurs in other tissues at lower levels. p38 gamma plays a mechanical role in response to stress signals by phosphorylating downstream targets affecting cellular responses such as proliferation and differentiation.
Biological function summary
P38 gamma/MAPK12 influences the regulation of gene expression apoptosis and cell cycle processes. It operates within a specific MAP kinase signaling module that conveys signals from the cellular surface to the nucleus. This protein sometimes works in conjunction with other members of the MAPK family forming complexes that further fine-tune cellular responses under various conditions. Its activities help cells adapt to changes in their environment by modulating transcription factors and other important substrates.
Pathways
The MAPK pathway and the stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) pathway both involve p38 gamma/MAPK12. Within these pathways p38 gamma is closely related to proteins such as JNK1 and JNK2 which also respond to stress signals. These pathways are essential for transmitting signals that regulate cellular growth differentiation and stress responses revealing intricacies of cellular adaptation to the environment.
Aberrant activation or dysfunction of p38 gamma/MAPK12 relates to conditions like cancer and cardiac hypertrophy. In cancer p38 gamma can influence tumor progression and metastasis through its impact on cell proliferation and survival. It also interacts with other proteins like MKK3 and MKK6 which serve as upstream activators in oncogenic signaling pathways. In cardiac hypertrophy altered p38 gamma signaling affects cardiac muscle cell growth potentially leading to heart failure. Understanding these connections provides insight into the mechanisms underlying these disorders.