Abcam, ab266283, Human TMEM14B knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
TMEM14B KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 19 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 19 bp deletion in exon 2

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TMEM14B, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TMEM14B also known as Transmembrane Protein 14B is a mitochondrial inner membrane protein with a molecular weight of approximately 15 kDa. It is involved in the transport of molecules across the mitochondrial membranes. TMEM14B is expressed in a variety of tissues with a significant presence in the respiratory and nervous systems. The protein comprises several transmembrane helices playing an important role in maintaining mitochondrial function.
Biological function summary
TMEM14B is key for mitochondrial biogenesis and the regulation of cellular energy metabolism. It is an integral component of the mitochondrial complex and contributes to the proper assembly of respiratory chain complexes. This protein facilitates the import of other essential proteins into the mitochondrial matrix ensuring efficient mitochondrial function and cellular energy homeostasis. Its activity is closely associated with energy production and apoptosis pathways.
Pathways
TMEM14B has essential roles in the oxidative phosphorylation and apoptosis pathways. These pathways are vital for energy production and programmed cell death respectively. TMEM14B's interaction with cytochrome c oxidase and caspases highlights its involvement in mitochondrial respiration and apoptotic regulation. Through these pathways TMEM14B collaborates with other mitochondrial proteins such as ATP synthase to support cellular energetic processes.
TMEM14B's dysfunction is linked to neurodegenerative disorders and metabolic syndromes. Its improper functioning may lead to impaired mitochondrial activity contributing to conditions like Alzheimer's disease and metabolic syndrome. The protein relation with cytochrome c in these disorders highlights TMEM14B's involvement in neuronal cell death and metabolic dysregulation. Understanding TMEM14B's role in these diseases can provide insights into potential therapeutic targets.