Abcam, ab266393, Human PINK1 knockout HEK-293T cell line

Size: 1000000Cells / vial / 2 x 1000000Cells / vial
PINK1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 10 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 10 bp deletion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PINK1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The PTEN-induced kinase 1 (PINK1) is a serine/threonine-protein kinase with a molecular weight of approximately 63 kDa. It plays a significant role in mitochondrial quality control through its kinase activity. PINK1 gets expressed in various tissues with a high presence in the brain. The protein localizes to the outer membrane of damaged mitochondria to initiate mitophagy an important cellular process for clearing dysfunctional mitochondria.
Biological function summary
The PINK1 protein detects mitochondrial damage and recruits Parkin an E3 ubiquitin ligase to the damaged mitochondria. This interaction leads to the ubiquitylation of mitochondrial substrates and triggers their degradation. PINK1 Parkin and other proteins form a complex that facilitates the removal of damaged mitochondria through autophagy. This mechanism ensures cellular health and energy balance by maintaining a pool of functional mitochondria.
Pathways
PINK1 integrates into the mitochondrial quality control and mitophagy pathways. It has a fundamental role in the PINK1-Parkin pathway which is critical for maintaining mitochondrial integrity. In this pathway PINK1 phosphorylates both ubiquitin and Parkin enhancing Parkin's E3 ligase activity. Another pathway that PINK1 participates in is the PTEN-induced kinase pathway where it regulates mitochondrial dynamics and homeostasis via cross-talk with other mitochondrial proteins such as DJ-1 and LRRK2.
PINK1 mutations are strongly associated with familial Parkinson's disease attributing to its role in preserving neuronal function through mitochondrial regulation. Deficient PINK1 function leads to accumulation of damaged mitochondria contributing to neuronal cell death. Additionally PINK1 dysfunction is implicated in Alzheimer's disease as impaired mitochondrial clearance is a contributing factor. Here the PINK1 interaction with other proteins like Parkin and DJ-1 highlights its importance in neurodegenerative disorders.