Abcam, ab266397, Human NTPCR (Nucleoside triphosphate phosphohydrolase) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
NTPCR KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 29 bp deletion in exon 1 and 2 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 29 bp deletion in exon 1 and 2 bp deletion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NTPCR, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Nucleoside triphosphate phosphohydrolase often called NTPase is an enzyme responsible for the hydrolysis of nucleoside triphosphates. It plays an important role in converting nucleoside triphosphates to nucleoside diphosphates with the release of inorganic phosphate. This process is important as it regulates the availability of nucleotides for various biological functions. NTPase typically with a mass of approximately 70 kDa is expressed in various tissues but shows high activity in tissues with rapid cell division. It is particularly studied in cellular lines such as HEK293T where it assists in the investigation of cellular mechanisms.
Biological function summary
This enzyme participates in critical cellular processes by managing the concentration of nucleoside triphosphates. Its activity influences DNA and RNA synthesis signal transduction and energy transfer. NTPase does not generally form complexes with other proteins but may interact with other molecules transiently during its catalytic action. This transient nature allows it to act efficiently where nucleoside triphosphate function is necessary.
Pathways
NTPase influences multiple biochemical pathways important for cell viability and function. It plays a role in the nucleotide metabolism pathway which is essential for DNA and RNA biosynthesis. Moreover its involvement in the energy transfer pathway ties it to proteins such as myosin and kinases which require energy from ATP hydrolysis to function properly. These interactions underline its importance in cellular physiology.
Disruptions in NTPase activity have been linked to mitochondrial disorders and certain cancers. Abnormal levels of nucleoside triphosphates caused by malfunctioning NTPase can lead to mitochondrial dysfunction contributing to disorders like mitochondrial myopathy. In cancer altered nucleotide metabolism may support uncontrolled cell proliferation. NTPase could connect with proteins such as p53 or oncogene products accumulating interest as a potential therapeutic target.