Abcam, ab266444, Human BLVRA (BVR) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
BLVRA KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 25 bp deletion in exon 3 and 7 bp deletion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 25 bp deletion in exon 3 and 7 bp deletion in exon 3

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-BLVRA, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The BVR protein known in full form as Biliverdin Reductase plays an essential mechanical role in the conversion of biliverdin to bilirubin. It exists as two main isoforms BVR-A and BVR-B with molecular masses approximately of 33 kDa. BVR is widely expressed in various tissues with higher levels found in liver kidney and spleen. As an enzyme its main function centers around the redox cycle between biliverdin and bilirubin providing an antioxidant defense mechanism.
Biological function summary
BVR influences cell signaling and growth processes acting as a multifunctional enzyme beyond its primary reductive activity. It participates in the MAPK and PI3K/Akt signaling pathways acting both as a kinase and phosphatase which affects transcription factor regulation. BVR is also involved in heme metabolism working in conjunction with heme oxygenase to recycle heme a vital cellular molecule.
Pathways
BVR significantly contributes to the antioxidative and anti-inflammatory pathways. It operates within the heme catabolic pathway and the MAPK signaling cascade linking to proteins like heme oxygenase-1 (HO-1) and the transcription factor Nrf2. These pathways support cell response to oxidative stress and regulate gene expression in response to environmental stressors.
BVR is associated with conditions such as jaundice and chronic inflammation-oriented diseases. Its interaction with proteins like heme oxygenase-1 (HO-1) elevates its importance in managing oxidative stress-related disorders. Disruption in BVR function or expression can influence bilirubin metabolism potentially leading to hyperbilirubinemia and contributing to inflammatory disease development through altered cell signaling.