Abcam, ab266458, Human SMARCD1 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
SMARCD1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 2 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 2 bp insertion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SMARCD1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SMARCD1 also known as SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1 is a component of the SWI/SNF chromatin remodeling complex. The protein has a mass of approximately 50 kDa. It shows expression in various tissues including the brain liver and heart. Alternative names for SMARCD1 include BAF60A indicating its role within the BAF (BRG1/BRM-associated factors) complex.
Biological function summary
SMARCD1 plays a significant role in altering chromatin structure to regulate gene expression. It functions as part of the SWI/SNF complex which modulates access of transcription factors to DNA. This protein contributes to the regulation of various genes involved in cellular processes like proliferation differentiation and DNA repair. The SWI/SNF complex including SMARCD1 alters nucleosome positioning affecting the transcriptional landscape.
Pathways
SMARCD1 influences the expression of genes involved in critical cellular activities. It participates actively in the Wnt signaling and the cell cycle regulation pathways. The Wnt pathway includes critical interactions with proteins like β-catenin which contributes to the regulation of gene transcription related to cell fate and proliferation. SMARCD1's involvement in cell cycle regulation also implicates interactions with proteins such as cyclins and p53.
The altered expression or mutation of SMARCD1 relates to conditions like cancer and developmental disorders. Overexpression of SMARCD1 has associations with certain types of cancers including breast cancer where SMARCD1 modulates interactions with key proteins like BRG1 and BRM. Developmental disorders may involve interactions affecting the SWI/SNF complex leading to issues in tissue differentiation and growth.