Abcam, ab266468, Human TMEM59 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
TMEM59 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TMEM59, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TMEM59 also known as Transmembrane Protein 59 plays a role in protein glycosylation and modulation of the cell surface expression of certain proteins. It has a molecular mass of approximately 39 kDa. TMEM59 is expressed in a variety of tissues including the brain lung and kidney where it localizes to the endoplasmic reticulum and Golgi apparatus. The protein's structure suggests membrane spanning properties that contribute to its function in cell regulation.
Biological function summary
TMEM59 contributes to proper neuronal function by modulating the cell surface expression of proteins involved in neuron-neuron communications. This regulation has implications for membrane protein function and synaptic stability. TMEM59 participates in certain bionetworks interacting with protein complexes that influence cell signaling pathways. Such interactions play significant roles in controlling protein trafficking and localization within cells impacting the cellular response to environmental signals.
Pathways
TMEM59 influences processes like the Wnt signaling pathway affecting cell fate decisions and embryonic development. It interacts with proteins such as β-catenin and affects their stability and function. TMEM59 also participates in the autophagy-dependent pathways modulating cellular mechanisms involved in the degradation of damaged proteins and organelles therefore maintaining cellular homeostasis.
TMEM59 has links to neurodegenerative diseases including Alzheimer's disease and schizophrenia. TMEM59's regulation of proteins like APP (Amyloid precursor protein) is significant. Disruptions in its function may contribute to aberrant protein aggregation commonly seen in Alzheimer's disease pathology. Additionally disruptions in TMEM59 expression or function could affect cognitive processes and synaptic connections related to schizophrenia as it influences the trafficking of receptor proteins important for synaptic signaling.