Abcam, ab266472, Human RAB1A knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
RAB1A KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 1 and 8 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 1 and 8 bp deletion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RAB1A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Rab1A also known as Ras-related protein Rab-1A is a small GTPase belonging to the Rab family. Rab1A has a role in regulating vesicle trafficking and it is involved in ER-to-Golgi transport. This protein with a molecular mass of approximately 23.5 kDa regulates intracellular traffic affecting vesicle budding motility and fusion. Rab1A is highly expressed in various tissues including the liver heart kidney and to lesser extents in others.
Biological function summary
The Rab1A protein influences intracellular transport processes and maintains cellular homeostasis. It forms part of multimeric protein complexes that facilitate its functions in the secretory pathway. The protein ensures the proper trafficking of cargo proteins from the endoplasmic reticulum to the Golgi apparatus playing a role in the early secretory pathway. This involvement is critical for maintaining the post-translational modification and processing of proteins.
Pathways
The mechanical function of Rab1A integrates into several cellular pathways including the secretory pathway and autophagy. Rab1A works with other proteins like Rab2 and Rab30 in the secretory pathway to coordinate the proper flow of proteins. Its coordination with proteins like LC3 during autophagy highlights its importance in cellular turnover and degradation processes linking Rab1A to essential biological functions and homeostasis.
Rab1A has a connection to certain cancers and neurodegenerative diseases. Aberrant Rab1A expression or mutations can lead to disruptions in cellular trafficking implicated in hepatocellular carcinoma. The protein's altered function could also influence the pathogenesis of Parkinson's disease with proteins such as α-synuclein showing interaction in disease pathways. Understanding these relationships helps in elucidating Rab1A's role in disease mechanisms.