Abcam, ab266492, Human NUP210 (GP210) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
NUP210 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 5 and 1 bp insertion in exon 5 and 28 bp deletion in exon 5. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 5 and 1 bp insertion in exon 5 and 28 bp deletion in exon 5

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NUP210, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GP210 also known as Nup210 is a critical component of the nuclear pore complex with a molecular mass of approximately 210 kDa. This integral membrane protein is primarily expressed in the nuclear envelope of eukaryotic cells. GP210 plays a significant mechanical role in the structural integrity of the nuclear pore complex a pivotal gateway for nucleocytoplasmic transport. Its localization at the nuclear envelope suggests its importance in maintaining the selective barrier and facilitating transport functions between the nucleus and the cytoplasm.
Biological function summary
GP210 is involved in the regulation of nuclear-cytoplasmic transport and acts as a scaffold for various transport machinery components. As a member of the nuclear pore complex it interacts with other nucleoporins forming a channel that allows the passage of molecules between the nucleus and cytoplasm. GP210 participates in processes related to genome regulation influencing gene expression by modulating nuclear import/export cycles. Alterations in GP210 function can have implications for the cell cycle and differentiation processes.
Pathways
GP210 engages in the nucleocytoplasmic transport pathway which is essential for the transport of macromolecules such as RNA and proteins. This pathway is closely related to the Ran GTPase cycle where GP210 plays a supporting role in maintaining the directionality of the transport. It interacts with transport factors such as importins and exportins ensuring the precise regulation of molecular traffic. GP210's involvement ties it to key cellular functions and highlights its critical role in cellular homeostasis and signal transduction.
Aberrant function or expression of GP210 has been implicated in autoimmune diseases such as primary biliary cholangitis (PBC). Autoantibodies against GP210 are often detected in patients with PBC indicating its role in the pathogenesis of this autoimmune liver disorder. Furthermore GP210 is associated with certain cancer types where its dysregulation might contribute to tumorigenesis and cancer progression. In these contexts GP210 could interact with oncogenic or tumor suppressor proteins highlighting its potential as a biomarker and target for therapeutic intervention.