Abcam, ab266497, Human NABP2 (hSSB1) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
NABP2 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 3

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NABP2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HSSB1 also known as OBFC2B is a single-stranded DNA-binding protein with a molecular mass of approximately 30 kDa. It is mainly expressed in the nucleus where it plays an important role in the maintenance and protection of genomic stability. The protein recognizes and binds to single-stranded DNA which is typical during DNA replication and repair processes. hSSB1 is highly conserved across different species highlighting its important function in cellular mechanisms.
Biological function summary
This protein plays a significant role in DNA damage response and repair. hSSB1 forms a complex with the INTS3 protein as part of the MRN (MRE11-RAD50-NBS1) complex to effectively respond to DNA double-strand breaks. This action is critical to initiate the appropriate signaling cascade that leads to DNA repair. The protein's expression and activity need tight regulation to ensure genome integrity and to prevent mutations.
Pathways
HSSB1 is involved in the homologous recombination repair pathway which is essential for error-free repair of DNA double-strand breaks. It interacts with BRCA1 and RAD51 which are key players in homologous recombination ensuring accurate DNA damage repair. Additionally hSSB1 participates in the ATR signaling pathway enhancing cell cycle checkpoint activation during replication stress where it collaborates with ATR and its substrates.
HSSB1 has been implicated in cancer development particularly in breast and ovarian cancers. Defects or dysregulation in hSSB1 may lead to inadequate DNA repair increasing the likelihood of mutations that contribute to carcinogenesis. Furthermore hSSB1 has connections to neurodegenerative disorders linked through its interactions with proteins like ATM. hSSB1's influence on maintaining genomic stability places it at the intersection of various pathways that affect cellular health and disease progression.