Abcam, ab266498, Human HINT2 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
HINT2 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon 1 and 1 bp insertion in exon 1 and 2 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon 1 and 1 bp insertion in exon 1 and 2 bp deletion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-HINT2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HINT2 also known as histidine triad nucleotide-binding protein 2 is a mitochondrial protein weighing approximately 17 kDa. It involves in catalyzing the hydrolysis of nucleotide derivatives specifically adenosine 5'-monophosphoramidate substrates. You would usually find HINT2 in the liver kidney and heart where it likely plays roles in cellular metabolism and energy homeostasis.
Biological function summary
This protein influences several cellular processes including apoptosis and mitochondrial function regulation. HINT2 is not part of a larger protein complex but works independently to ensure proper mitochondrial activity. Through these actions HINT2 maintains cellular homeostasis which is important for healthy energy production and utilization within the cell.
Pathways
This protein interacts with key signaling pathways such as the mitochondrial apoptosis pathway. This pathway usually relates to programmed cell death and links closely with proteins like cytochrome c and caspases. By influencing this pathway HINT2 plays a part in determining cell fate and contributes to the balance between cell survival and death.
Research links HINT2 to metabolic syndromes and liver diseases. Alterations in this protein may affect how cells manage energy which could lead to or exacerbate conditions like non-alcoholic fatty liver disease. The relationship of HINT2 with transporter proteins such as carnitine palmitoyltransferase 1 suggests it might influence fatty acid metabolism providing insights into how energy imbalances can trigger or worsen metabolic issues.