Abcam, ab266531, Human RCN2 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
RCN2 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 2 bp insertion in exon 1 and 37 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 2 bp insertion in exon 1 and 37 bp deletion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RCN2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Reticulocalbin 2 (RCN2) also known as E6-binding protein and ERC-55 is a calcium-binding protein with a mass of approximately 39 kDa. It localizes primarily to the endoplasmic reticulum where it helps in protein folding and processing. RCN2 is broadly expressed in many tissue types notably in the brain heart and liver. Functionally RCN2 contains EF-hand motifs that bind calcium ions which is important for its role in maintaining calcium homeostasis within cells.
Biological function summary
This protein interacts with cellular mechanisms associated with calcium signaling pathways. RCN2 forms part of a larger molecular complex that influences protein processing in the endoplasmic reticulum. As a chaperone it helps in ensuring that proteins achieve their correct conformations. Research shows that RCN2 also interacts with various cell signaling molecules and contributes to the regulation of cellular stress responses.
Pathways
Involvement of RCN2 extends to the unfolded protein response (UPR) and calcium signaling pathways. In the UPR RCN2 helps maintain endoplasmic reticulum function and protein folding. RCN2 interacts with significant pathway proteins such as GRP78/BiP which is an important regulator in maintaining protein-folding homeostasis. In calcium signaling the regulation by RCN2 supports intracellular calcium balance and communication between organelles.
Researchers have linked RCN2 to conditions such as neurodegenerative disorders and cardiovascular diseases. Altered expression of RCN2 has been noted in Alzheimer's disease potentially impacting calcium signaling and protein misfolding. Additionally RCN2 interacts with proteins involved in heart conditions such as SERCA2 affecting calcium uptake in cardiac cells. Understanding RCN2's roles may provide insights into therapeutic approaches for these disorders.