Abcam, ab266585, Human PTPRF (LAR) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
PTPRF KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 4 and 5 bp deletion in exon 4. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 4 and 5 bp deletion in exon 4

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PTPRF, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein LAR also known as Leukocyte Common Antigen-Related phosphatase is a receptor-like protein tyrosine phosphatase. It weighs approximately 220 kDa. LAR is expressed in various tissues including the nervous system and digestive tract. It comprises an extracellular domain a single transmembrane domain and two intracellular phosphatase domains which play a significant role in its interaction with other proteins.
Biological function summary
This receptor-like phosphatase supports important cell signaling functions. LAR is involved in neural development processes and is part of the synaptic adhesion complex. By interacting with proteins in the plasma membrane it helps modify and maintain neural connectivity necessary for proper nerve function. Its activity influences actin cytoskeleton dynamics and cell-cell adhesion.
Pathways
LAR plays a role in the axon guidance and insulin signaling pathways. In axon guidance it interacts with proteins such as netrin and nneural cell adhesion molecule (NCAM) to facilitate correct neural wiring. In the insulin signaling pathway LAR can dephosphorylate key proteins like insulin receptor affecting glucose metabolism and overall energy homeostasis.
Research shows that LAR may be linked to diabetes and potential neurological disorders. In diabetes its interaction with the insulin receptor suggests a role in insulin resistance which is a major contributing factor in the disease. In neurological disorders LAR's dysregulation can affect neural development and function possibly linking it to diseases like schizophrenia where cell signaling and connectivity changes are observed.