Abcam, ab266633, Human TMEM208 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
TMEM208 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 41 bp insertion in exon 1 and 7 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 41 bp insertion in exon 1 and 7 bp deletion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TMEM208, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TMEM208 also known as transmembrane protein 208 plays a role in cellular processes involving the endoplasmic reticulum and lysosomes. With an estimated molecular weight of around 24 kDa scientist can find TMEM208 predominantly expressed in human tissues particularly in the heart and skeletal muscle. As a multi-pass membrane protein TMEM208 establishes the cell organelles' communication ensuring intracellular stability and proper functioning of cellular processes.
Biological function summary
The protein serves an essential function in the formation and maintenance of autophagic vesicles. It operates as a component of the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) participating in cargo sorting and transport. TMEM208's role in vesicle nucleation is important for autophagy a process where cells remove unnecessary or dysfunctional components maintaining cellular homeostasis. This action indirectly supports cellular adaptation to stress.
Pathways
TMEM208 integrates into intracellular trafficking and autophagy pathways working alongside proteins such as LC3 and ATG-related proteins. Through its involvement in vesicular transport TMEM208 collaborates in the lysosomal degradation pathway a process important for recycling cellular materials. TMEM208's interaction with LC3 connects it to the macroautophagy pathway allowing the cell to respond effectively to nutrient deprivation or other cellular stressors.
TMEM208 associates with pathologies linked to improper autophagic processes and cellular stress responses including neurodegenerative diseases like Alzheimer's and possibly myopathies. Researchers note its association with proteins such as p62 which accumulates when autophagy is disrupted indicating TMEM208's potential role in disease progression. Understanding TMEM208's function might lead to identifying therapeutic targets for these conditions.