Abcam, ab266663, Human RBM3 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
RBM3 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 2

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RBM3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RBM3 also known as RNA-binding motif protein 3 is a cold-inducible protein with a mass of approximately 17 kDa. It plays a role in binding RNA and is widely expressed in tissues including the brain heart and skeletal muscle. Research often highlights its involvement in temperature response implicating its role in cellular adaptation to cold stress. Overexpression of RBM3 has been documented during hypothermic conditions which suggests an adaptive function in extreme environments.
Biological function summary
RBM3 functions by modulating RNA stability and translation impacting cellular stress responses. It forms part of ribonucleoprotein complexes influencing the processing of several RNA species. The protein enhances mRNA stability under stress conditions and promotes cell survival by impacting protein synthesis. RBM3 also participates in synaptic plasticity which is essential for learning and memory showcasing its extensive influence on cellular functioning.
Pathways
RBM3 significantly contributes to neuroprotection and anti-apoptosis pathways. It is closely linked to pathways dealing with stress granules and mRNA stabilization. A known association exists between RBM3 and proteins such as CIRP (cold-inducible RNA-binding protein) which share similar pathways concerning mRNA regulation under cold conditions. This connection highlights RBM3's role in cell survival and stress management.
RBM3 has shown connections to neurodegenerative diseases and cancer. High levels of RBM3 expression correlate with improved outcomes in hypothermia-treated stroke and trauma patients due to its neuroprotective abilities. In cancer RBM3 links with proteins such as HIF-1α (hypoxia-inducible factor-1 alpha) where its overexpression has been associated with tumor progression in certain types suggesting RBM3's dual protective and pathological roles depending on the disease context.