Abcam, ab266708, Human CECR5 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
HDHD5 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon 6 and 1 bp deletion in exon 6. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon 6 and 1 bp deletion in exon 6

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-HDHD5, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CECR5 also known as Cat Eye Syndrome Critical Region Candidate 5 encodes a protein with a mass of about 48 kDa. This protein shows expression in multiple tissues such as the brain kidney and liver. Its mechanical role involves binding interactions that help in signaling and cellular communication processes. The protein's structure enables it to participate effectively in these interactions making it a point of interest for further study in these specific tissues.
Biological function summary
CECR5 serves an important function in cellular signaling networks and is not typically found as part of a larger complex. The protein plays a role in modulating certain cellular responses possibly impacting processes like differentiation and growth. Researchers have identified CECR5 involvement in cellular mechanisms that govern normal development and maintenance hinting at its broader influence in cellular homeostasis.
Pathways
CECR5 engages in the MAPK signaling pathway which is instrumental in transferring extracellular signals to intracellular responses. The MAPK pathway involves proteins like ERK and JNK with which CECR5 may interact or have regulatory effects. Additionally it influences signaling via the PI3K-AKT pathway a pathway that controls many aspects of cell survival growth and metabolism. This association suggests CECR5's potential roles in important cellular activities and regulatory mechanisms.
Alterations in CECR5 expression or function relate to Cat Eye Syndrome and possibly neurodevelopmental disorders. In Cat Eye Syndrome the overexpression or structural changes of CECR5 may disrupt normal development alongside chromosomal duplications involving the region. Connections also appear between CECR5 and other proteins like PAX6 in the context of its role in developmental processes. Understanding these relationships further explains CECR5's impact on these conditions.