Abcam, ab266725, Human RAB6A (Rab-6) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
RAB6A KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RAB6A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Rab-6 also known as Ras-related protein 6 acts as a GTPase involved in membrane trafficking. This protein has a molecular mass of approximately 24 kDa. Researchers find Rab-6 expressed in various tissues with high levels in the brain and lungs. It localizes primarily to the Golgi apparatus where it participates in the regulation of intracellular transport.
Biological function summary
Rab-6 aids in the coordination of retrograde vesicle transport from the Golgi to the endoplasmic reticulum. Rab-6 functions as part of a complex with other proteins including dynactin and cytoplasmic dynein to facilitate these movements. Its role includes ensuring proper delivery and sorting of proteins within the cell which is essential for maintaining cellular organization and function.
Pathways
This protein is involved in the endocytosis and secretory pathways. In the endocytosis pathway Rab-6 interacts with proteins like Rab GDI and Rab escort protein to regulate vesicle transport. In the secretory pathway the protein ensures that cargo proteins transit correctly through the Golgi interacting with other Rab family members such as Rab-1 which also play parts in vesicle movement and secretion processes.
Rab-6 has been implicated in neurological disorders and certain cancers. For instance abnormalities in Rab-6 function have been linked to Alzheimer's disease where it associates with proteins like amyloid precursor protein affecting its processing. Additionally altered Rab-6 activity can contribute to cancer metastasis through interactions with metastasis-related proteins which influences cell migration and invasion mechanisms.