Abcam, ab266727, Human PRDX4 (Peroxiredoxin 4) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
PRDX4 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PRDX4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Peroxiredoxin 4 also known as Prdx4 is a protein that acts as an antioxidant enzyme and belongs to the peroxiredoxin family. It influences the reduction of hydrogen peroxide and organic hydroperoxides. The molecular mass of Prdx4 is approximately 31 kDa. It is expressed mainly in the endoplasmic reticulum but you can find some expression in the cytosol as well.
Biological function summary
Prdx4 plays a list of roles in cellular defense against oxidative stress. It not only reduces peroxides but also contributes to the maintenance of protein folding by modifying disulfide bonds in the endoplasmic reticulum. Prdx4 does not function as part of a larger complex but acts independently within its compartments. The protective functions of Prdx4 are important for cell survival and proper cellular function.
Pathways
Prdx4 interacts with oxidative stress response pathways. Specifically it participates in the redox signaling pathways that regulate cellular reactive oxygen species levels. Peroxiredoxin 4 is closely associated with proteins such as thioredoxin in these pathways. Together they regulate the cellular redox environment which is important for maintaining cellular homeostasis.
Oxidative stress imbalance implicates Prdx4 in conditions like diabetes and cancer. For example in diabetes altered Prdx4 levels disturb normal cell function due to increased oxidative stress. In cancer Prdx4 can influence tumor progression by modulating the antioxidant capacity within cells. Prdx4 also shows a functional connection to proteins like glutathione peroxidase in regulating oxidative stress-related pathologies.