Abcam, ab266736, Human SSR4 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
SSR4 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 1 and 8 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 1 and 8 bp deletion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SSR4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SSR4 also known as TRG4 or CDG1S is a subunit of the signal recognition particle receptor responsible for the translocation of proteins across the membrane of the endoplasmic reticulum. With an approximate mass of 24 kDa SSR4 is expressed predominately in tissues with high secretory functions such as the liver and pancreas. It plays an important role in facilitating the proper insertion and folding of nascent polypeptide chains by cooperating with other components of the translocation machinery.
Biological function summary
SSR4 functions as part of the signal sequence receptor (SSR) complex. This complex is essential for the targeting and translocation of newly synthesized proteins into the endoplasmic reticulum lumen. SSR4 interacts directly with signal sequences of nascent polypeptides to ensure their correct orientation and localization during protein synthesis. Proper functioning of SSR4 is necessary for the homeostasis of the cellular secretory pathway.
Pathways
SSR4 is involved in the protein translocation pathway which is essential for protein sorting and trafficking. This pathway intersects with the unfolded protein response (UPR) pathway which maintains protein homeostasis under stress conditions. In these pathways SSR4 collaborates with proteins like Sec61 to mediate translocation and assist in protein folding. Disruptions in these pathways can lead to cellular dysfunction and stress-related responses.
Defects or alterations in SSR4 are linked to congenital disorders of glycosylation (CDGs) which affect multiple organ systems. Altered glycoprotein production in these disorders results from impaired protein translocation demonstrating SSR4's influence on glycoprotein processing. Furthermore SSR4 may interact with proteins like glycosyltransferases affecting their function in cellular processes and contributing to the broad clinical spectrum observed in CDGs.