Abcam, ab266804, Human SLC5A6 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
SLC5A6 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 3 and 7 bp deletion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 3 and 7 bp deletion in exon 3

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SLC5A6, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SLC5A6 also known as the sodium-dependent multivitamin transporter (SMVT) mechanically functions as a transmembrane protein responsible for the uptake of vital water-soluble vitamins and cofactors such as biotin pantothenic acid and lipoate. The protein has an approximate molecular mass of 72 kDa. It is expressed in various tissues with high levels observed in the intestines kidneys and placenta indicating its role in nutrient absorption and metabolic processes.
Biological function summary
SLC5A6 is essential for the efficient transport of vitamins across cell membranes using sodium gradient as the driving force. It transports vitamins into cells where they participate as coenzymes in several enzymatic reactions. This transporter does not form part of a larger protein complex but functions individually to link dietary intake with cellular nutrient requirements. The absorption through SLC5A6 impacts various biosynthetic processes as biotin and pantothenate contribute to fatty acid synthesis and energy metabolism.
Pathways
The role of SLC5A6 in nutrient absorption connects it to critical metabolic pathways such as the biotin and CoA biosynthetic pathways. Its function impacts cellular metabolism by providing essential cofactors for carboxylation reactions and energy production. SLC5A6 interacts with enzymes that rely on these vitamin-derived cofactors maintaining cellular metabolic equilibrium essential for cell growth and energy balance.
Mutations or dysfunction in SLC5A6 can lead to conditions like biotinidase deficiency which results in various neurological and dermatological symptoms. There are connections between SLC5A6 malfunctions and certain metabolic disorders due to impaired nutrient uptake linking this transporter to proteins involved in biotin metabolism. Research indicates possible associations of SLC5A6 with neurological disorders as biotin deficiency impacts brain functions and development.