Abcam, ab266841, Human EIF3K knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
EIF3K KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 64 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 64 bp deletion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-EIF3K, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EIF3K also known as eukaryotic translation initiation factor 3 subunit K is a protein subunit of the eIF3 complex. This protein has a mass of approximately 30 kilodaltons and it is expressed in many tissues across the body. The mechanical role of eIF3K is primarily in the assembly and functioning of the translation initiation complex which positions ribosomes at the start codon of messenger RNA (mRNA) strands to begin protein synthesis.
Biological function summary
EIF3K is involved in the regulation of the eukaryotic translation initiation factor 3 complex which is essential for initiating translation by serving as a scaffold for other eIF3 subunits. This complex stabilizes the formation of the pre-initiation complex necessary for ribosome binding to mRNA. Through its involvement in protein synthesis eIF3K influences cell growth proliferation and response to various cellular signals. It is involved in processes such as cell cycle control and apoptotic regulation as it aids the translation of specific mRNAs coding for key regulatory proteins.
Pathways
EIF3K participates in mTOR and Wnt signaling pathways. These pathways are critical in controlling cell growth proliferation and differentiation. Through mTOR signaling eIF3K interacts with proteins like ribosomal protein S6 kinase beta-1 (S6K1) influencing cell metabolism and growth. In the Wnt pathway eIF3K contributes to the translation of proteins that regulate cell fate and stem cell renewal. Interactions with these pathways illustrate the importance of eIF3K in maintaining cellular homeostasis.
Alterations in eIF3K function have links to cancer and neurodegenerative diseases. In cancer dysregulated eIF3K expression can lead to unregulated cell growth and tumorigenesis due to its role in protein synthesis and cell proliferation. eIF3K connects to oncogenic proteins such as c-Myc which stimulate cancer progression. In neurodegenerative diseases eIF3K may contribute to cell death through its interactions with apoptotic pathways and impaired cellular stress responses highlighting its potential as a target for therapeutic intervention.