Abcam, ab266957, Human IFIT1 knockout A549 cell line

Size: 1000000Cells / vial / 2 x 1000000Cells / vial
IFIT1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 3 and 25 bp deletion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 3 and 25 bp deletion in exon 3,
Disease:Carcinoma

Product details:
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-IFIT1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
IFIT1 also known as Interferon-Induced Protein with Tetratricopeptide Repeats 1 is a protein that plays a significant role in antiviral defense mechanisms. It has a molecular mass of approximately 56 kDa. Researchers find IFIT1 predominantly expressed in cells stimulated by interferons. The protein detects viral single-stranded RNA which in turn helps initiate the antiviral response. You will find IFIT1 in various cell types mainly those exposed to viral infections as it is an important part of the body's innate immune system.
Biological function summary
Activities of IFIT1 focus on inhibiting viral replication and translation processes. The protein binds to viral RNAs via its distinctive sensor domains preventing infections from progressing. IFIT1 operates often as part of a larger protein complex with other IFIT family members improving its ability to intercept and inhibit viral RNA. This concerted action provides an important line of defense against diverse viral pathogens by interfering with viral protein synthesis.
Pathways
IFIT1 occupies an important place in the interferon signaling pathways particularly the Jak-STAT pathway. This pathway is critical for mounting antiviral responses when viral infections occur. Through its interactions within the pathway IFIT1 works together with related proteins like IFIT2 and IFIT3 to repel viral threats. By integrating into these pathways it contributes to amplifying the immune response promoting a robust defense system against foreign viral invaders.
IFIT1 is associated mainly with viral diseases such as influenza and hepatitis C. Its role in these diseases involves preventing the communication and replication of the viruses making it a pivotal part of antiviral therapeutic research. Additionally proteins like RIG-I interact with IFIT1 during these processes to recognize viral RNA enhancing the effectiveness of the immune response. These protein partnerships drive ongoing research looking to leverage IFIT1's antiviral properties for treatment developments.