Abcam, ab266986, Human LAP3 knockout A549 cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
LAP3 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp insertion in exon 6. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp insertion in exon 6,
Disease:Carcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-LAP3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LAP3 also known as leukotriene A4 hydrolase has a molecular weight of approximately 53 kDa. This enzyme functions as an aminopeptidase which is a type of enzyme that removes amino acids from the amino terminus of proteins and peptides. It is expressed in many tissues with significant levels found in the liver and kidney. LAP3 also shows activity in the cellular cytoplasm indicating it plays a role in cellular metabolic processes.
Biological function summary
LAP3 participates in protein maturation and degradation. It plays a role in processing peptides that are destined for further degradation or cellular signaling. The LAP3 protein is not known to be part of a larger complex but it works alongside other enzymes involved in peptide metabolism. This activity contributes to the regulation of intracellular peptide concentrations and supports cellular homeostasis.
Pathways
LAP3 is linked to important biological processes such as protein catabolism and the regulation of blood pressure. It interacts within the peptide processing pathways to influence the generation of bioactive peptides. LAP3 works in conjunction with other proteolytic enzymes such as aminopeptidases and endopeptidases which are essential for various cellular functions. Through these pathways LAP3 indirectly impacts the regulation of physiological processes.
LAP3 is associated with hypertension and certain metabolic disorders. It influences conditions like high blood pressure through its role in generating peptides that can modulate vascular functions. LAP3 has connections with proteins involved in these conditions such as angiotensin-converting enzyme (ACE) which also plays a part in blood pressure regulation. Understanding the functions of LAP3 aids in delineating its potential as a therapeutic target for these diseases.