Abcam, ab266988, Human PLSCR1 (Scramblase 1) knockout A549 cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
PLSCR1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 4 and 1 bp insertion in exon 4. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 4 and 1 bp insertion in exon 4,
Disease:Carcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PLSCR1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Scramblase 1 also known as PLSCR1 is a protein involved in the translocation of phospholipids between the inner and outer leaflets of the lipid bilayer. This protein has a molecular mass of approximately 37 kDa. PLSCR1 is found in various tissues and cells with notable expression in the hematopoietic system. As a type II membrane protein Scramblase 1 plays a role in maintaining lipid asymmetry especially during cellular activation or apoptosis.
Biological function summary
Scramblase 1 mediates phospholipid scrambling in response to increased intracellular calcium levels facilitating changes in cell membrane morphology. It performs this function independently and as part of specific protein complexes that regulate membrane curvature and vesicle fusion. The role of PLSCR1 extends beyond membrane activities; it has been linked to signal transduction and the immune response suggesting a broader impact on cellular dynamics.
Pathways
Scramblase 1 influences critical biological processes like blood coagulation and apoptosis. It interacts with proteins such as annexin V which stabilizes membrane phospholipids and regulates cell death pathways. In the coagulation cascade PLSCR1 has been shown to interact with factors involved in platelet activation demonstrating its regulatory role in maintaining hemostasis and tissue homeostasis.
Scramblase 1 has notable implications in cancer and autoimmune diseases. Altered expression or mutation of PLSCR1 links directly to the progression of certain cancers often associating with proteins like p53 which plays an important role in cell cycle regulation and tumor suppression. In autoimmune disorders Scramblase 1's activity affects the balance of immune cell signaling potentially linking it with proteins involved in inflammatory responses such as cytokines. Understanding these connections provides insights into potential therapeutic targets and diagnostic markers for these diseases.