Abcam, ab267023, Human IFIT5 knockout A549 cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
IFIT5 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 2 and 311 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 2 and 311 bp deletion in exon 2,
Disease:Carcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-IFIT5, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
IFIT5 also known as Interferon-induced protein with tetratricopeptide repeats 5 is a protein with a molecular mass of approximately 56 kDa. This protein is expressed mainly in the cytoplasm where it functions by specifically binding to single-stranded RNAs lacking 2'-O-methylation at their 5'-cap structure. This recognition allows IFIT5 to distinguish self from non-self RNA an important element in immune defense mechanisms. Its ability to sense and bind to viral RNA makes it an important player in the host's innate immune response.
Biological function summary
IFIT5 is involved in antiviral defense and immune response regulation. It does not function as part of a multi-protein complex but interacts with various signaling molecules to amplify immune responses. By selectively binding to foreign RNA IFIT5 can inhibit viral replication and modulate type I interferon signaling pathways. It plays a significant role in restricting viral protein synthesis giving the host organism time to mount an adaptive immune response.
Pathways
IFIT5 is closely associated with the interferon signaling pathway and the innate immune response pathway. Through its interactions it affects the Jak-STAT signaling pathway which is important for the induction of antiviral states in cells. IFIT5 also has functional links with other proteins such as RIG-I an important sensor in the detection of viral RNAs. Together they provide a robust early immune defense by facilitating cytokine production and ensuring rapid response to infections.
IFIT5 has been linked to viral infections and certain inflammatory conditions. Its role in antiviral defense connects it to diseases such as hepatitis C where the protein's influence on interferon action can affect viral persistence and patient response to treatment. Additionally IFIT5 interaction with proteins like PKR moves it to the context of autoimmune disorders where overactive or dysregulated immune responses can lead to chronic inflammation and tissue damage.