Abcam, ab267033, Human KDM7A (JHDM1D) knockout A549 cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
KDM7A KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 5 and 7 bp deletion in exon 5 and 8 bp deletion in exon 5. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 5 and 7 bp deletion in exon 5 and 8 bp deletion in exon 5,
Disease:Carcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-KDM7A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
JHDM1D also known as KDM7A is a histone demethylase enzyme involved in the removal of methyl groups from di- and trimethylated lysine residues on histone tails particularly at H3K9 and H3K27. This action impacts gene expression by altering chromatin structure making it an important player in regulating transcriptional activities. The target has a molecular mass of approximately 129 kDa. JHDM1D is widely expressed in various tissues with notably high expression levels in the brain and testis indicating its importance in these organs.
Biological function summary
JHDM1D functions as part of a larger protein complex that regulates chromatin remodeling and gene expression. It plays a significant role in epigenetic modification processes influencing cell differentiation and development through its demethylation activity. Such capabilities affect cellular proliferation and lineage specification and may impact immune responses and neurological functions.
Pathways
Epigenetic regulation and chromatin remodeling see direct involvement from JHDM1D through pathways including the histone modification pathway and Wnt signaling pathway. In these pathways JHDM1D interacts with related proteins like EZH2 which also takes part in chromatin modifications and transcriptional control. This interplay highlights the importance of JHDM1D in fine-tuning gene expression.
Altered JHDM1D function relates to cancer progression and multiple neurological disorders. Dysregulation of this protein may contribute to abnormal cell growth and development as seen in certain cancers. Additionally its interactions with proteins like p53 suggest a potential role in tumor suppression pathways. In neurological disorders JHDM1D's impact on brain-specific gene expression may underline deficits seen in conditions like autism spectrum disorders.