Abcam, ab267059, Human HAPLN3 knockout A549 cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
HAPLN3 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3,
Disease:Carcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-HAPLN3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HAPLN3 also known as hyaluronan and proteoglycan link protein 3 mechanically acts to stabilize the extracellular matrix structure by binding hyaluronic acid and proteoglycans. This protein has a mass of about 38 kDa. HAPLN3 is notably expressed in tissues like cartilage and brain contributing to its functional roles in these areas. Among its alternate names cartilage link protein 3 is recognized underlining its connection with connective tissues.
Biological function summary
Hyaluronan and proteoglycan link protein 3 integrates into the extracellular matrix by forming part of a complex network essential for tissue structure and integrity. HAPLN3 contributes to the stabilization of matrix macromolecules directly influencing cell adhesion migration and proliferation. It ensures the structural rigidity necessary for normal tissue functions and provides the necessary dynamic balance for tissue repair and maintenance.
Pathways
Hyaluronan and proteoglycan link protein 3 participates in the formation and remodeling of the extracellular matrix pathway important for processes like embryogenesis and wound healing. It interacts with proteins such as aggrecan and versican which are also key components of the matrix regulatory network. Additionally HAPLN3 is involved with the hyaluronan metabolism pathway assisting in maintaining homeostasis of tissue hydration and elasticity.
Hyaluronan and proteoglycan link protein 3 has associations with osteoarthritis where its expression affects cartilage degradation and regeneration. It is also connected to neurological disorders due to its expression in the brain where it may interact with neurocan. Aberrant regulation or mutation of HAPLN3 can lead to disrupted extracellular matrix integrity contributing to the pathophysiology of these conditions.