Abcam, ab267161, Human PARP10 (ARTD10) knockout A549 cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
PARP10 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 5 and 1 bp insertion in exon 5 and 4 bp deletion in exon 5. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 5 and 1 bp insertion in exon 5 and 4 bp deletion in exon 5,
Disease:Carcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PARP10, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein ARTD10 also known as PARP10 is a member of the poly(ADP-ribose) polymerase (PARP) family. It exhibits a mass of approximately 100 kDa. ARTD10 acts mechanically as mono-ADP-ribosyltransferase which modifies target proteins by adding ADP-ribose units. This specific post-translational modification controls protein function and interactions. ARTD10 expression occurs mostly in the cytoplasm and nucleus across different cell types indicating its versatile functional roles.
Biological function summary
ARTD10 influences several cellular processes including transcriptional regulation and cell cycle progression. It functions independently without being part of a stable protein complex but interacts with other nuclear and cytoplasmic proteins to exert its role. This protein is involved in modulating cellular responses to DNA damage and stress impacting cellular homeostasis and metabolism. Its interaction patterns highlight its significance in cellular regulatory mechanisms.
Pathways
ARTD10 is an active participant in DNA repair and stress response pathways. It is closely linked with the NF-kB signaling pathway where it modulates inflammation and cell survival outcomes. ARTD10 also connects with proteins like PARP1 sharing a broader role in the maintenance of genomic integrity and response to DNA insults. These pathways underpin the critical mechanisms by which cells manage and adapt to damages and maintain cellular viability.
ARTD10's dysregulation associates with certain cancers and inflammatory conditions. Aberrant activity of ARTD10 impacts cellular proliferation and apoptosis linking it with tumorigenesis. Inflammation-related disorders also see a correlation with altered ARTD10 activity. This protein remains related to key proteins like NF-kB in understanding its role in these diseases as they collectively influence inflammatory and tumor progression pathways.