Abcam, ab267220, Human PARP12 knockout A549 cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
PARP12 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3,
Disease:Carcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PARP12, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PARP12 or poly (ADP-ribose) polymerase family member 12 plays a role in post-translational modifications within cells. It functions in mono-ADP-ribosylation a process which affects various proteins' activities by adding ADP-ribose to them. This protein has a calculated mass of approximately 78 kDa. It expresses in various tissues including the liver and immune cells contributing to cellular responses and defense mechanisms.
Biological function summary
Mono-ADP-ribosylation modulates cellular activities affecting processes like viral response and inflammation. PARP12 is part of the broader PARP protein family known for roles in DNA repair and cellular stress responses though PARP12 focuses more on antiviral and stress-related pathways. It does not typically engage in large multi-protein complexes but interacts closely with other molecules within its signaling network to execute its functions.
Pathways
Interactions within antiviral defense mechanisms and inflammation regulation highlight the role of PARP12. It contributes to pathways like the Interferon signaling response enhancing the cell's ability to combat viral infections. Interactions with other PARP family members such as PARP1 and PARP5 further integrate its function into broader cellular immune pathways ensuring a coordinated response to external stressors.
PARP12's involvement predominantly impacts viral infections and inflammatory conditions. It relates to viral response pathways such as those engaged in HIV replication repression highlighting its significance in infectious disease contexts. Furthermore PARP12 connects with proteins like PARP13 in modulating processes involved in immune responses offering insights into managing inflammation-related diseases.