Abcam, ab267244, Human UQCRQ knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
UQCRQ KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 3

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-UQCRQ, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The UQCRQ protein also known as the ubiquinol-cytochrome c reductase complex III subunit VII has a mass of approximately 9.5 kDa. It is a component of the mitochondrial respiratory chain complex III. This protein is expressed in various tissues throughout the body with a higher concentration in tissues that require substantial energy production. UQCRQ plays a mechanical role in the electron transport chain facilitating the transfer of electrons between coenzyme Q and cytochrome c. This process contributes to the proton gradient used by ATP synthase for ATP synthesis.
Biological function summary
UQCRQ is a part of the cytochrome bc1 complex which is a critical enzyme in the mitochondrial inner membrane. This complex is essential in cellular respiration helping in the conversion of energy from nutrients into usable cellular energy in the form of ATP. The activity of UQCRQ is important for maintaining the efficiency of this larger complex and any disruption can significantly affect cellular energy homeostasis. UQCRQ supports the integrity of mitochondrial function impacting both energy production and reactive oxygen species management.
Pathways
UQCRQ is pivotal in oxidative phosphorylation and the electron transport chain specifically within the mitochondrial respiratory complex III. It works closely with other proteins in the complex such as cytochrome b and cytochrome c1 ensuring efficient electron transfer. Through these pathways UQCRQ contributes to oxidative stress regulation and cellular energy metabolism linking energy-producing mechanisms to broader cellular processes like apoptosis and cell growth.
UQCRQ has associations with mitochondrial disorders and myopathies. Mutations or dysfunctions in UQCRQ can lead to impaired energy production manifesting in conditions like mitochondrial encephalomyopathy and hypertrophic cardiomyopathy. The dysfunction in UQCRQ can affect proteins such as cytochrome c which plays a role in apoptosis potentially leading to neurodegenerative changes seen in mitochondrial-related diseases. Understanding the implications of UQCRQ in these conditions can guide the development of therapeutic strategies targeting mitochondrial dysfunction.