Abcam, ab267328, Human GK (Glycerol kinase) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
GK KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 4. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 4

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-GK, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glycerol kinase also known as GK or Gyk acts as a catalyst in the phosphorylation of glycerol to glycerol-3-phosphate. This enzymatic activity is important for the utilization of glycerol in metabolic processes. Glycerol kinase is expressed in tissues such as liver kidney and adipose tissue. Its molecular weight varies depending on the species but in humans it is around 60 kDa when analyzed using methods like glycerol SDS PAGE. In human cells like HEK293T reliable detection of glycerol kinase is possible due to its considerable expression.
Biological function summary
The main role of glycerol kinase relates to its function in lipid metabolism and energy homeostasis. It exists as a part of a biochemical pathway but does not typically form a complex with other proteins. Glycerol kinase enables cells to utilize glycerol as an energy source by converting it into glycerol-3-phosphate which feeds into further metabolic pathways. This conversion is essential for the balance of energy supply and the production of triglycerides and phospholipids.
Pathways
Metabolism and energy production heavily rely on the function of glycerol kinase. This enzyme acts within the glycerolipid metabolism and gluconeogenesis pathways. In these pathways it is connected to other enzymes like glycerol-3-phosphate dehydrogenase which further processes the glycerol-3-phosphate produced by glycerol kinase. The enzyme's availability affects the rate of these pathways and in turn influences lipid and glucose metabolism in the body.
Glycerol kinase deficiency leads to complex metabolic syndromes such as hyperglycerolemia and congenital adrenal hyperplasia. Hyperglycerolemia results from excess glycerol in the blood due to impaired conversion by the enzyme. In congenital adrenal hyperplasia alterations in metabolic function relate to glycerol kinase's impact on stress response and energy balance. Disorders related to glycerol kinase sometimes involve interactions with other proteins and enzymes involved in broader metabolic processes affecting overall metabolism and physiology.