Abcam, ab267586, Rat Agrin ELISA Kit

Size: 1 x 96Tests
Rat Agrin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat Agrin in Rat in Cell Culture Media, Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell culture media, EDTA Plasma, Heparin Plasma, Serum,
Reacts with:Rat,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.06 ng/mL,
Range:0.069 - 50 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Rat Agrin ELISA Kit is designed for the quantitative determination of Agrin in cell culture supernatants, plasma and serum samples.
Δ Note:
Collect plasma samples using Heparin or EDTA as anti-coagulant. Citrate is not recommended plasma samples.
This assay employs an antibody specific for rat Agrin coated on a 96-well plate. Standards and samples are pipetted into the wells and Agrin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat Agrin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Agrin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: Rat Activin A, beta-NGF, CINC-1, CINC-2, CINC-3, CNTF, Fractalkine, IL-1alpha, IL 1beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3alpha, MMP-8, PDGF-AA, TIMP-1, TNF-alpha, VEGF.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Agrin also known as AGRN is a large heparan sulfate proteoglycan with a molecular mass of approximately 200 kDa. This protein is mainly expressed in the neuromuscular junction and the central nervous system. Agrin has significant roles in synapse formation. It influences the clustering of acetylcholine receptors at the neuromuscular junction which is essential for communication between nerve and muscle cells. This activity is mechanically mediated through interactions with the receptor tyrosine kinase MuSK and LRP4.
Biological function summary
The Agrin protein contributes to the stabilization of synaptic structures. Its ability to induce acetylcholine receptor clustering is fundamental for the proper formation and maintenance of neuromuscular synapses. Agrin is not a lone player; it is a part of a larger signaling complex. This complex includes other proteins like MuSK which acts as a receptor playing an essential role in complex signaling pathways that regulate synaptogenesis and synaptic plasticity.
Pathways
Many molecules associate to facilitate communication and synapse integrity via Agrin's activity. Agrin engages primarily with the MAPK/ERK signaling pathway and the PI3K/AKT pathway. These pathways are central for cell growth and differentiation in the central nervous system. The relationship with MuSK through these pathways highlights Agrin's importance in orchestrating the synapse assembly and stability.
Mutations or alterations in Agrin can lead to conditions such as congenital myasthenic syndromes and potentially impact neurodegenerative disorders like amyotrophic lateral sclerosis. In these cases its interaction with proteins like MuSK is affected leading to impaired neuromuscular transmission. Understanding the anti-Agrin antibody's function might enhance methodologies to treat these neuromuscular disorders.