Abcam, ab267595, Human IL-24 ELISA Kit

Size: 1 x 96Tests
Human IL-24 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-24 in Human in Cell Culture Media, Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.41 ng/mL,
Range:0.41 - 100 ng/mL,
Assay Platform:Microplate

Product details:
Human IL-24 ELISA Kit is designed for the quantitative determination of IL-24 in cell culture supernatants, plasma and serum samples.
Δ Note:
Human IL-24 concentration is low in normal serum/plasma, and may not be detectable in this assay. Collect plasma using EDTA or Citrate as an anticoagulant. Heparin is not recommended.
This assay employs an antibody specific for human IL-24 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-24 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-24 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-24 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Less than 0.2% cross-reactivity with mouse IL-24 is observed.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C