Abcam, ab267608, Human Gas 1 ELISA Kit

Size: 1 x 96Tests
Human Gas 1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Gas 1 in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 45 pg/mL,
Range:46.88 - 3000 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Gas 1 ELISA Kit is designed for the quantitative determination of Gas 1 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human Gas 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Gas 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Gas 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Gas 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The growth arrest-specific 1 protein or Gas1 plays an inhibitory role in cell cycle progression by interacting with various growth factors and receptors. Gas1 often termed as Gas-1 is a 38 kDa protein. It expresses prominently in quiescent cells particularly in the nervous and embryonic tissues. This expression helps control the proliferation of cells which is critical for maintaining normal cellular functions and structure.
Biological function summary
Gas1 acts as a negative regulator of cell proliferation particularly during developmental processes. It is not part of a complex but serves as a standalone inhibitor that influences cell growth. The protein interacts with components like Hedgehog signaling which leads to its involvement in developmental signaling pathways. Gas1's role in growth arrest demonstrates its importance in controlling the balance of cellular differentiation and proliferation.
Pathways
Gas1 significantly influences the Hedgehog signaling pathway and the G1 phase of the cell cycle. In the Hedgehog pathway Gas1 interacts with other key proteins like Patched and Smoothened regulating the pathway's activity which is essential for embryonic development and tissue patterning. Its interaction with these proteins highlights its regulatory function in signal transduction influencing how cells respond to growth cues.
Gas1's dysregulation links to cancer and congenital malformations. Such disorders often occur due to alterations in the Hedgehog signaling pathway implicating Gas1 as a contributor in aberrant cell growth conditions. In cancer specifically Gas1 may influence tumor suppression mechanisms impacting cells' growth arrest. Mutations in components of the Hedgehog pathway such as Smoothened can alter Gas1's function giving rise to developmental disorders and cancer progression.