Abcam, ab267615, Human Siglec 7 ELISA Kit

Size: 1 x 96Tests
Human Siglec 7 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Siglec 7 in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture media, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.1 ng/mL,
Range:0.102 - 25 ng/mL,
Assay Platform:Microplate

Product details:
Human Siglec 7 ELISA Kit is designed for the quantitative determination of Siglec 7 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human Siglec 7 coated on a 96-well plate. Standards and samples are pipetted into the wells and Siglec 7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Siglec 7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Siglec 7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAMTS13, Aggrecan, Angiotensinogen (SERPINA8), B7-H1 (CD274), BMPR-IA (ALK-3), BMPR-II, Cadherin-11, CD27 (TNFRSF7), CD6, Ck beta 8-1 (CCL23), CNTF, DNAM-1 (CD226), CD147 (EMMPRIN), FLRG, Follistatin-like 1, Fractalkine (CX3CL1), Galectin-1, GITR Ligand (TNFSF18), Granulysin (NKG5/LAG-2), IL-1 R3 (IL-1 R Acp), IL-15 R alpha, IL-17E (IL-25), IL32 alpha, CHL-1 (L1CAM-2), LRIG3, LRP-6, MEPE (OF45), Nectin-4, Periostin, Persephin, Renin, RGM-B, ROBO3, S100 A8, Syndecan-3, Thrombospondin 2, Thrombospondin 5 (COMP), Tie-1, ULBP-2 (ALCAN-alpha/N2DL2).

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C