Abcam, ab267648, Human BMP9 ELISA Kit

Size: 1 x 96Tests
Human BMP9 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human BMP9 in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 32 pg/mL,
Range:32.77 - 8000 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human BMP9 ELISA Kit is designed for the quantitative determination of BMP9 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human BMP9 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BMP9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Activin A, aFGF, Amphiregulin, bFGF, BMP-4, E-Selectin, ICAM-1, IGF-1, IL-1 alpha, IL-1 beta, IL-6, IL8, IL-11, IL-17A, MCP-1, M-CSF, MIP-1 alpha, MMP-2, MMP-9, MMP-13, Osteoactivin, PCadherin, RANK, SDF-1 alpha, Shh-N, TGF beta 1, TGF beta 2, TNF alpha, VCAM-1, VECadherin.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C