Abcam, ab268398, Recombinant human Cdk7 + Cyclin H/p34 protein (Active)

Size: 5µg
Recombinant human Cdk7 + Cyclin H/p34 protein (Active) is a Human Full Length protein, in the 1 to 323 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>70% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:Tag free,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity of ab268398 was 3.0 nmol/min/min in a kinase assay using myelin basic protein as substrate.,
Accession:P50613,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7Preservative: 1.02% ImidazoleConstituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

Product details:
Proteins co-expressed.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cdk7 also known as Cyclin-dependent kinase 7 forms a complex with Cyclin H sometimes referred to as p34. Mechanically Cdk7 functions as a kinase an enzyme that adds phosphate groups to other proteins which is an important regulatory activity in many cellular processes. As a part of the CDK-activating kinase (CAK) complex Cdk7 has a molecular mass of around 40 kDa. It resides in various cellular compartments predominantly in the nucleus where its regulatory activity largely occurs.
Biological function summary
The Cdk7-Cyclin H/p34 complex plays a significant role in cell cycle regulation acting at important checkpoints for transitioning between different phases of the cell cycle. It participates in the phosphorylation of other cyclin-dependent kinases therefore activating them and promoting cell cycle progression. The Cdk7-Cyclin H complex is an essential factor of the transcription process as it forms part of the transcription factor IIH (TFIIH) a multiprotein complex involved in transcription initiation and DNA repair.
Pathways
Cdk7 is an important regulator within the cell cycle and transcription regulation pathways. Notably it acts in close coordination with the p53 protein during DNA damage response ensuring genomic integrity through controlled cell cycle arrest. Additionally Cdk7 associates with the MAPK pathway linking signal transduction with cell cycle control which further exemplifies its broad regulatory impact within cells.
Dysregulation of the Cdk7-Cyclin H/p34 complex is associated with cancer particularly in its ability to alter cell cycle control and transcription regulation which may lead to unchecked cell proliferation. Studies have shown that cancer cells often exhibit elevated levels of Cdk7 linking its activity to oncogenic transformation. Moreover variations in Cdk7 activity are implicated in neurodegenerative diseases particularly Alzheimer's disease where altered transcriptional regulation contributes to disease pathogenesis. The p53 protein which interacts with Cdk7 often shows mutations in cancer highlighting their interconnected roles in these conditions.