Abcam, ab268535, Recombinant Red fluorescent protein eqFP611 (His tag)

Size: 20µg
Recombinant Red fluorescent protein eqFP611 (His tag) is a Entacmaea quadricolor Full Length protein, in the 1 to 231 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q8ISF8,
Animal free:No,
Carrier free:No,
Species:Entacmaea quadricolor,
Storage buffer:pH: 7Preservative: 1.02% ImidazoleConstituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The red fluorescent protein eqFP611 often referred to by its sequence identifiers like e q f p 6 1 1 is a significant protein in scientific research. This protein with a mass of around 26.6 kDa originates from the sea anemone Entacmaea quadricolor. Scientists express it in various systems for its bright fluorescence distinct from the green and other less red-shifted proteins. EqFP611's properties make it an invaluable tool for imaging applications especially in live cell imaging where visualization of cellular structures is necessary.
Biological function summary
EqFP611 serves as a marker for visualizing and tracking cellular processes under a microscope. It does not naturally occur as part of human cellular complexes but researchers commonly use it to tag other proteins. Its fluorescence helps localize the proteins in their cellular environments providing insights into protein movements interactions and functions. The protein's fluorescent capabilities allow scientists to study dynamic biological processes in vivo with minimal interference.
Pathways
EqFP611 is largely utilized outside of natural cellular pathways as a tool for research rather than a participant. Biochemically it does not engage directly in pathways such as MAPK or PI3K but rather aids in observing them by tagging proteins involved in these pathways. EqFP611 helps in deciphering signaling pathways by marking proteins like Raf kinase and AKT which play roles in cell growth and survival.
EqFP611 is integral to studying pathological processes especially in cancer research. Researchers use it to observe tumor growth and metastasis in real time often tagging tumor-related proteins like phosphoinositide-dependent kinase-1 (PDK1) in cancer models. Although eqFP611 itself does not contribute to diseases it is important in identifying and tracking disease progression. It assists in uncovering the interactions and roles of different proteins implicated in disorders such as cancer and neurodegeneration.