Abcam, ab268639, Recombinant human HIPK2 protein (Active)

Size: 5µg
Recombinant human HIPK2 protein (Active) is a Human Fragment protein, in the 1 to 640 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:GST tag N-Terminus,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity of ab268639 was determined to be 130 nmol/min/mg in a kinase assay using MBP substrate.,
Accession:Q9H2X6,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HIPK2 also known as Homeodomain-interacting protein kinase 2 is a serine/threonine kinase with a molecular mass of approximately 118 kDa. This protein plays a mechanical role in modulating transcriptional regulation and apoptosis. It interacts with homeodomain transcription factors influencing the activity of various gene promoters. Within cells HIPK2 is expressed widely in several tissues including the heart brain and liver highlighting its diverse functional involvement across different biological systems.
Biological function summary
The wide-ranging actions of HIPK2 make it essential for cell growth apoptosis and stress response. HIPK2 associates with various transcription factors and other-binding partners forming dynamic complexes that mediate its functions. By phosphorylating target proteins it regulates p53 and promotes p53-mediated apoptosis making it an important part of stress response networks. Additionally HIPK2 is implicated in the regulation of Wnt signaling pathways which are important for cell proliferation and differentiation processes.
Pathways
HIPK2 plays an integrative role in apoptotic and Wnt signaling pathways. It serves as a facilitator of the DNA damage response primarily through the modulation of p53 activity. It also contributes to the Wnt signaling pathway by phosphorylating beta-catenin and enhancing its degradation therefore regulating cellular proliferation. The interactions of HIPK2 with proteins like p53 and beta-catenin illustrate its significant involvement in controlling cell fate and maintaining cellular homeostasis.
HIPK2 is linked to various cancers and neurodegenerative diseases due to its role in apoptosis and cell cycle regulation. Disruption in HIPK2 activity leads to impaired apoptosis and contributes to tumorigenesis particularly in cancers like breast and colorectal cancer. In neurodegenerative disorders such as Alzheimer's disease abnormal HIPK2 activity is associated with altered cellular signaling. The relationship with key proteins like p53 further explains how deviations in HIPK2 function can lead to disease states making it a valuable target for therapeutic interventions.