Abcam, ab268789, Recombinant human METTL3 + METTL14 protein (Active)

Size: 5µg
Recombinant human METTL3 + METTL14 protein (Active) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:Tag free,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity of ab268789 was 750 pmol/min/mg in an in vitro assay using an oligo ssRNA substrate.,
Accession:Q86U44,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Product details:
Proteins co-expressed

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
METTL3 and METTL14 are important players in RNA methylation specifically involved in N6-methyladenosine (m6A) modification. METTL3 often referred to as methyltransferase-like 3 has a molecular weight of approximately 70 kDa and frequently forms a complex with METTL14 in cells. The METTL3 protein is mainly expressed in the nucleus where it orchestrates the process of adding a methyl group to adenosine residues on RNA transcripts. This activity modifies mRNA molecules influencing their stability and translation efficiency.
Biological function summary
METTL3 and METTL14 function together as the core components of the m6A methyltransferase complex which also includes associated proteins like WTAP. This complex plays a significant role in gene expression and RNA metabolism. METTL3 and METTL14 regulate mRNA splicing nuclear export and degradation by acting on specific consensus sequences. Recognition of these sequences allows for the modulation of various RNA biological processes impacting cellular response and development.
Pathways
METTL3 and METTL14 play key roles in the m6A RNA methylation pathway influencing signaling pathways such as the mTOR and Wnt pathways. These pathways are vital for regulating cell proliferation and differentiation. Additionally METTL3 interacts with proteins like YTHDF1 which act as m6A readers and help mediate the effects of methylated RNA on biological outcomes creating a link between m6A marks and the functional response within the cell.
METTL3 and METTL14 have shown associations with certain cancers and neurological disorders. In various cancers such as acute myeloid leukemia upregulated METTL3 levels correlate with increased malignancy and poor prognosis. In neurological contexts altered m6A modification patterns from METTL3 and METTL14 activity may connect to proteins like FTO which has implications in neurodevelopmental disorders. Therefore understanding their roles in these conditions offers potential therapeutic avenues.