Abcam, ab268808, Recombinant human N6AMT2 protein (Active)

Size: 5µg
Recombinant human N6AMT2 protein (Active) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:His tag N-Terminus,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity of ab268808 was 110 pmol/min/mg in a methyltransferase assay using histone H4 peptide (1-21) as substrate.,
Accession:Q8WVE0,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7Preservative: 1.02% ImidazoleConstituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
N6AMT2 also known as N-6 adenine-specific DNA methyltransferase 2 is a protein responsible for methylating adenine residues in DNA. This post-translational modification involves the transfer of a methyl group to specific DNA sequences affecting gene expression and genomic stability. N6AMT2 weighs approximately 50 kDa and expresses widely in various human tissues including the liver brain and kidneys.
Biological function summary
N6AMT2 influences chromatin structure and gene regulation through its methyltransferase activity making it an important player in epigenetic regulation. It does not act in isolation but associates with other methyltransferases and chromatin-modifying complexes enhancing its function in modifying gene expression patterns. These associations allow N6AMT2 to regulate the accessibility of DNA to transcription factors and other DNA-binding proteins.
Pathways
N6AMT2 participates in the DNA methylation pathway integral for controlling gene expression and maintaining DNA integrity. It works closely with other DNA methyltransferases like DNMT1 to ensure the proper methylation marks are set and maintained during DNA replication. Furthermore N6AMT2 engages in the histone modification pathway where it collaborates with histone methyltransferases to coordinate chromatin remodeling and transcriptional regulation.
The aberrant activity of N6AMT2 associates with certain cancers such as hepatocellular carcinoma and glioblastoma. It influences tumorigenesis through its role in DNA methylation and chromatin remodeling which can lead to altered gene expression profiles. N6AMT2 has direct interactions with oncogenic pathways and protein partners like EZH2 a histone-lysine N-methyltransferase highlighting its importance in cancer progression and offering a potential target for therapeutic intervention.