Abcam, ab268819, Recombinant human GST-Nucleoplasmin ALK (mutated F1174L) fusion protein (Active)

Size: 5µg
Recombinant human GST-Nucleoplasmin ALK (mutated F1174L) fusion protein (Active) is a Human protein, expressed in Sf9 cells, with >70%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>70% SDS-PAGE,
Expression system:Sf9 cells,
Tags:GST tag N-Terminus,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity of ab268819 was determined to be 8 nmol/min/mg in a kinase assay using IGF1Rtide synthetic peptide substrate.,
Accession:Q9UM73,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Preservative: 1.02% ImidazoleConstituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

Product details:
GST-Nucleoplasmin-ALK (F1174L) fusion protein.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ALK-Nucleophosmin fusion protein also known as NPM-ALK is an oncogenic fusion resulting from a translocation between the NPM1 gene and the ALK gene. The native ALK protein short for Anaplastic Lymphoma Kinase is a receptor tyrosine kinase that weighs approximately 220 kDa. It is normally expressed in the central nervous system testes and small intestine. The fusion with NPM1 a protein of around 38 kDa commonly found in the nucleolus results in a chimeric protein exhibiting constitutive kinase activity that contributes to oncogenesis.
Biological function summary
The ALK-Nucleophosmin fusion protein disrupts normal cellular signaling processes. It acts by activating downstream signaling pathways that promote cellular proliferation and survival. NPM1 besides its role in ribosome biogenesis aids in the transport of proteins and ribonucleic acids. When fused with ALK the NPM1 segment facilitates the relocation of the ALK portion leading to aberrant activation of signaling cascades. The fusion protein forms part of oligomeric complexes that overshadow the regular cell regulatory mechanisms.
Pathways
ALK-Nucleophosmin fusion protein plays a significant role in the PI3K/AKT and RAS/MAPK pathways. The fusion's constitutively active kinase domain causes persistent activation of PI3K/AKT promoting cell growth and anti-apoptotic signals. Also its impact on RAS/MAPK engages proliferative responses. In doing so it interacts with proteins like GRB2 and SOS1 instrumental in mediating these signaling pathways. This integration into critical pathways highlights its pivotal role in pathogenesis.
The ALK-Nucleophosmin fusion protein is prominently associated with anaplastic large cell lymphoma (ALCL) and other non-Hodgkin lymphomas. Its presence is a defining characteristic of ALCL driving the malignancy through sustained proliferative signaling. Additionally researchers find a link between this fusion and certain cases of lung cancer. The role of other proteins such as STAT3 is critical in this context as the ALK-Nucleophosmin fusion protein activates them further propagating oncogenic signals. The understanding of this fusion protein ties directly into therapeutic strategies targeting the abnormally activated pathways and interacting proteins.