Abcam, ab268858, Recombinant human PI 3 K p85 alpha + PI 3 K catalytic subunit alpha/PIK3CA (E1021K) protein

Size: 5µg
Recombinant human PI 3 K p85 alpha + PI 3 K catalytic subunit alpha/PIK3CA (E1021K) protein is a Human Full Length protein, in the 1 to 1044 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:Tag free,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity of ab268858 was 2,500 nmol/min/mg in a kinase assay using a PI(4,5)P2:PS mixture as substrate.,
Accession:P27986,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7Preservative: 1.02% ImidazoleConstituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

Product details:
Subunits co-expressed.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The PI 3 Kinase p85 alpha also known as PIK3R1 and PI 3 Kinase catalytic subunit alpha (PIK3CA) functions as an important component in cellular signal transduction. These proteins form a Class IA PI 3-kinase complex where p85 alpha serves as the regulatory subunit and p110 alpha acts as the catalytic subunit. PIK3CA the catalytic subunit weighs approximately 124 kDa. These proteins are expressed in various tissues with high expression in adipose tissue liver and the brain playing a significant role in mediating cell growth proliferation and metabolism.
Biological function summary
These proteins operate as part of a lipid kinase complex. They phosphorylate the inositol ring of phosphoinositides at the 3-position important in the generation of lipid second messengers. The active PI 3-kinase complex primarily phosphorylates phosphatidylinositol (PI) phosphatidylinositol 45-bisphosphate (PI(45)P2) creating phosphatidylinositol 345-trisphosphate (PI(345)P3) which then propagates intracellular signaling cascades. This activity regulates various cellular processes including survival and migration.
Pathways
PI 3 Kinase p85 alpha and PIK3CA are integral to the PI3K/AKT/mTOR signaling pathway. This pathway plays a pivotal role in regulating cell cycle and is intricately involved in pathways that control cellular quiescence proliferation cancer and longevity. The pathway's components such as AKT and mTOR are critical effectors of PI3K signaling. Additionally the proteins interrelate with other pathways through interactions with proteins like PTEN which acts as a negative regulator by dephosphorylating PI(345)P3.
These components have significant associations with oncogenic processes due to mutations in PIK3CA that are frequently observed in various cancers including breast and colorectal cancer. Such mutations lead to constitutive activation of the PI3K/AKT pathway promoting tumorigenesis. The proteins also link to insulin resistance-related disorders as enhanced PI3K activity affects metabolic signaling. Disorders like these connect through interactions with proteins such as IRS-1 which becomes dysregulated in the context of these diseases further highlighting their implication in aberrant signaling pathways.