Abcam, ab268888, Recombinant human CARM1 protein (Active)

Size: 5µg
Recombinant human CARM1 protein (Active) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:GST tag N-Terminus,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity of ab268888 was 800 pmol/min/mg in a methyltransferase assay using histone H3 peptide as substrate.,
Accession:Q86X55,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CARM1 also known as PRMT4 is a protein with a mass of approximately 65 kDa. It belongs to the protein arginine methyltransferase (PRMT) family of enzymes. CARM1 modifies histones by methylating arginine residues which plays a role in regulating transcription. This protein expresses widely across diverse tissues but shows higher levels in the lung thymus and spleen. These expression patterns suggest its broad function in modulating gene expression within specialized tissues.
Biological function summary
CARM1 serves as an important regulator of transcriptional activation. It can act independently or as part of a multiprotein complex to enhance transcription factor activity including steroid hormone receptors. In addition to histones CARM1 targets non-histone proteins like p300/CBP which affects their coactivator functions. It has a significant impact on gene expression by altering chromatin structure and accessibility thereby influencing cellular processes like differentiation and proliferation.
Pathways
One can find CARM1 involved in pathways controlling gene expression and cell cycle regulation. The protein is particularly important in the estrogen receptor signaling pathway where it interacts with the estrogen receptor alpha (ERα) and modulates transcriptional activation of estrogen-responsive genes. It also plays a role in the p53 signaling pathway by enhancing p53-mediated transcriptional activation linking CARM1 to cell cycle arrest and apoptosis. Both pathways highlight its importance in cellular growth and homeostasis.
Abnormalities in CARM1 function connect to specific cancers including breast and prostate cancer. Its deregulation leads to altered methylation patterns that can drive cancer progression and metastasis. CARM1 influences the expression and activity of proteins such as cyclin D1 which affects cell cycle progression and tumor growth. Understanding the involvement of CARM1 in these disorders opens potential therapeutic avenues for targeting its aberrant pathways in cancer treatment.