Abcam, ab268979, Recombinant human SPHK2 protein (Active)

Size: 5µg
Recombinant human SPHK2 protein (Active) is a Human Full Length protein, in the 1 to 654 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:GST tag N-Terminus,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity of ab268979 was 150 nmol/min/mg in a kinase assay using sphingosine as substrate.,
Accession:Q9NRA0,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SPHK2 also known as sphingosine kinase 2 is an enzyme responsible for the conversion of sphingosine to sphingosine-1-phosphate (S1P). This enzyme weighs approximately 68 kDa and is expressed in a wide range of tissues including liver kidney and brain. The catalytic activity of SPHK2 involves the phosphorylation of sphingosine which plays a part in the modulation of various cellular processes.
Biological function summary
SPHK2 influences cell growth survival and differentiation by generating sphingosine-1-phosphate a signaling lipid. It functions independently or in association with other proteins but it is not always a part of a larger complex. The generated S1P acts as a signaling molecule both inside and outside of cells affecting processes like apoptosis and inflammation.
Pathways
SPHK2 is a significant player in the sphingolipid signaling pathway and impacts the MAPK/ERK pathway. These pathways collectively regulate cellular proliferation and apoptosis. SPHK2 works in conjunction with SPHK1 a closely related sphingosine kinase in these signaling routes. These kinases together help to balance the levels of sphingosine and S1P which is essential for maintaining cellular homeostasis.
SPHK2 has been linked to cancer and inflammatory diseases. The overexpression of SPHK2 can result in increased levels of sphingosine-1-phosphate promoting cancer cell survival and resistance to chemotherapy. In addition SPHK2's involvement in inflammation suggests roles in conditions like rheumatoid arthritis where it might interact with TNF-α related signaling pathways. These connections highlight SPHK2 as a potential target for therapeutic intervention in such diseases.